Detection of invA Gene in Isolated Salmonella from Poultry carcass by PCR Method

Salmonella is a leading cause of worldwide food borne illness. Isolations ofSalmonella are reported more often from poultry carcass and poultry products.Salmonella contamination in poultry products is primarily due to cross contamination byphysical contact during carcass processing such as improper cleaning and disinfection ofprocessing lines, improper chilling and storage temperature. The presence of Salmonellawas detected in 60 samples of poultry carcasses from retail shops in Sanandaj, iran. Thepresence of Salmonella was assessed by conventional culture method and confirmed byPCR method. PCR amplification of InvA gene as a specific method for detection ofSalmonella was evaluated. In this study Salmonella was isolated from 16.66% of samplesby conventional culture method and confirmed by PCR. This study showed a widespreadprevalence of salmonella in poultry carcasses from local store in Sanandaj, iran