Zymogram Analysis of Alkaline Keratinase Produced by Nitrogen Fixing Bacillus pumilus ZED17 Exhibiting Multiprotease Enzyme Activities
Message:
Abstract:
Background
One group of significant enzymes produced by Bacillus genus are alkaline proteases with several important applications in the daily life as well as common industries such as food, detergents, leather, alcohol and beer production and medical and sanitary industries, beside wastewater treatment,, biotransformation, hydrolyzed proteins and oil manufacturing..
Objectives
In this paper, keratinase activity and zymogram analysis of Bacillus pumilus ZED17 using different conditions and substrates are reported..
Materials And Methods
The nitrogen fixing Bacillus, obtained from heated see water, was enriched on feather as the only sources of carbon, nitrogen and energy. It was also determined whether the isolated nitrogen fixing Bacillus exhibited extracellular proteolytic activity on feather, meat, gelatin and casein. Biochemical tests, carbohydrate fermentation patterns and 16srRNA detection were employed for identification of the isolated strain. Furthermore, the extracellular proteolytic activity using different protein substrates was investigated..
Results
B. pumilus ZED17 is one of the best strains enriched on feather, of which the extracellular proteolytic activities are exhibited. Activity-pH profiles were resoluted in buffers with different pH levels. Extracellular enzyme activities were assayed using different proteins and feathers. Keratinase activity was observed at neutral and alkaline, but not low pH levels. This enzyme demonstrated to have multi–activity. zymogram test revealed a 50-kD Caseinase produced by this strain. The optimum keratinase activity was at pH 8.0 and 40°C, using keratin as a substrate..
Conclusions
Since the isolated strain is halotolerant and nitrogen fixing, it is a good candidate for alkaline protease production, and soil fertilizing, in addition to biofertilizer production out of poultry and fish byproduct..
Language:
English
Published:
Jundishapur Journal of Microbiology, Volume:6 Issue: 10, Dec 2013
Page:
7974
magiran.com/p1210580  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 990,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
دسترسی سراسری کاربران دانشگاه پیام نور!
اعضای هیئت علمی و دانشجویان دانشگاه پیام نور در سراسر کشور، در صورت ثبت نام با ایمیل دانشگاهی، تا پایان فروردین ماه 1403 به مقالات سایت دسترسی خواهند داشت!
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 50 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!