The Effect of Glutamine Supplementation and Interval Exercise on HSP72 and CK Response in Soccer Players

The purpose of this study was to determine the effect of short-term consumption of Glutamine supplement and interval exercise on serum HSP72 and CK response in soccer players. For this reason, 29 soccer players (mean age, 19.3±4 years; weight, 69.3±2 kg; height, 176.6±1.2 cm; body fat percentage, 12.1±1.7; body mass index, 22.1±2.3 kg/m2; Vo2max, 53.5±1.3 kg.ml-1min-1) Voluntary were from Tehran Railway players and randomly divided into four groups of control (n=7), supplement (n=7), supplement- exercise (n=8) and exercise (n=7). All subjects have participated in daily soccer workouts before the study and only supplement-exercise and exercise groups did interval exercise. Blood samples of basal (12-14 hours after overnight fasting), pre test, post test and 90 minutes after test were taken from antecubital vein of non-dominant hand. Supplement (Glutamine) and placebo (dextrin) with amount of 0/5 g/kgBW and 5 ml/kgBW respectively, were consumed in one hour before interval exercise protocol. The interval protocol consisted of three sets of running with 80% of maximum heart rate and rest times with 5 minutes walking between them. Hsp72 and Ck values were measured using Elisa and Enzymatic methods respectively. For data analyzing in four groups in 4 stages of test, analysis of variance with repeated measures test and factor between groups and bonferoni post hoc at P≤0/05 were used. The results show that Hsp72 values 21% (P=0/001) at post test in supplement group and 16% (P=0/001) at post test in supplement- exercise group and 41% (P=0/001) at 90 minutes after exercise stage in supplement-exercise group has increased compared to the basal stage. Also Hsp72 values between post test stage in supplement group compared to post test stage in control group, has increased 15% (P=0/047). Hsp72 values between post test stage in supplement-exercise group compared to post test stage in control group has increased 20% and Hsp72 values between 90 minutes after test stage in supplement-exercise group compared to 90 minutes after exercise stage in control group has increased 40% (P=0/033). Hsp72 values have increased in supplement group at post test stage (P≤0/001) and in supplementexercise group at post test stage (P≤0/001) and 90 minutes after exercise (P≤0/001) compared to basal level. Analysis of the results derived from the study show that in the subjects athlete glutamine supplementation may be effective in stimulating of Hsp72 response and interval exercise had no effective role in hsp72 response.