The Effects of Vitamin C and E Supplementation and Anaerobic Activity on Oxidative Indices in Male Teenager Speed Skaters

Balance between free radical production and body antioxidant is disrupt by exercise trainings and cause a condition known as oxidative stress. The purpose of this study was to assess the effects of chronic (14 days) vitamins C, E supplementation on Malondialdehyde (MDA), plasma antioxidant enzymes, glutathione peroxidase (GPX), catalase (CAT) and total antioxidant capacity(TAC) in trained speed skaters. Eighteen male speed skaters with two years of background of competition were recruited (14.0±1.8 yrs, 161 ± 7.03 cm, 57.0 ± 6.4 kg) and divided in a double-blind manner randomly to placebo group (400 mg of a dextrose, 250ml water) and supplement group (200 IU vitamin E and 400mg vitamin C) for 14-days.Both groups performed 1000m speed skate test and Blood samples were taken be-fore the test. Second trial has been repeated after 14 days supplementation and same training protocol in same manner and blood samples has been taken before, after, 30-min subsequent the related test. The data were analyzed using analysis of variance (ANOVA) with repeated measure at P<0.05 in SPSS18 The results showed that the level of MDA and activity of GPX, CAT decreased significantly and TAC increased with 14 days supplementation after speed skate test. Therefore, two weeks vitamin C and vitamin E supplementation, the degenerative effect of oxidants on plasma membrane were probably deceased and the activity of antioxidant enzymes decreased in speed athletes.