THE EFFECTS OF CRYOPRESERVATION AND LYOPHILIZATION ON ENDOTHELIAL CELLS ADHESION TO HUMAN AMNIOTIC MEMBRANE
Message:
Abstract:
Background and Aims
Human amniotic membrane has some specific properties making it an appropriate biomaterial for using in vascular tissue engineering. In this study، amniotic membrane was preserved with different methods. Effects of preservation on amniotic extracellular matrix and adhesion of cultured endothelial cells to membrane were compared with fresh samples of amniotic membrane.
Materials and Methods
Human amniotic membrane was preserved with cryopreservation (-80 ̊C for 12 month) or lyophilization methods. Extracellular matrix components were assayed with immunohistochemistry method. The adhesion of cultured endothelial cells was studied with MTT assay. Results between groups were compared with ANOVA (Post-test Tukey).
Results
Results demonstrated that extracellular matrix components were same in cryopreserved samples in comparison to fresh ones but there are some differences in lyophilized samples. Adhesion of endothelial cells to lyophilized samples was significantly more than cryopreserved or fresh samples (P Value < 0. 05).
Conclusion
Both cryopreservation and lyophilization affect extracellular matrix of human amniotic membrane which can determine the rate of the adhesion of endothelial cells to amniotic membrane. Lyophilized amniotic membrane is a better choice for culture of endothelial cells in vascular tissue engineering.
Language:
Persian
Published:
Journal of Medical Science Studies, Volume:24 Issue: 10, 2014
Pages:
753 to 762
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