Cloning and sequence analysis of VP1, VP2 and VP3 genes of Indian chicken anemia virus

Chicken anemia virus was detected by PCR in tissue samples collected from poultry flocks in Gujarat، India. The VP1، VP2 and VP3 gene sequences of CAV from Anand، Gujarat were obtained after cloning the PCR products in pDrive cloning vector. Nucleotide sequence alignment with other CAV sequences demonstrated overall identity of 95-98. 8%، 98. 8-99. 8% and 98. 8-100% for VP1، VP2 and VP3 regions، respectively. Deduced amino acid sequences revealed 91. 7-99. 7%، 99-100% and 97. 3 100% homologies for VP1، VP2 and VP3 proteins، respectively، indicating high level of genome conservation. Further، placement of critical nucleotides and amino acids at particular positions indicated that Anand CAV is possibly of more pathogenic potential. The CAV isolates were phylogenetically grouped together independent of their geographic origin.