The effect of honey bee venom on the differentiation potency of D-alpha tocopheryl succinate (vitamin E) on HL-60 promyelocytic leukemia cell line

Acute promyelocytic leukemia is the most malignant type of myeloid leukemia characterized by chromosomal translocation (15 and 17) and also blocking the cells in promyelocytic stage of differentiation into myeloid. Nowadays، differentiation therapy is used to treat leukemia. Previous studies indicate that vitamin E inhibits proliferation and also induces differentiation of HL-60 cell line towards monocyte. Since high concentrations of vitamin E to induce differentiation have many side effects، the search for alternative compounds is inevitable. Regarding anti- proliferative and anti-cancer effect of bee venom (BV)، in this study the effect of BV on alpha tocopheryl succinate function in differentiation was examined. In this study cellular differentiation was tested by immunocytochemistry، Wright-Giemsa staining and NBT reduction. Data were analyzed using one-way ANOVA test and Instate 3 software. The results showed that BV in non-toxic concentrations can increase the differentiation potency of vitamin E on HL-60 cancer cell line. Non- toxic concentration of BV can increase differentiational effects of vitamin E and it is expected that BV can increases the differentiating potential of differentiator components in the future.