Study of therapeutic and histopathologic effects of corn silk's aqueous and metanolic extract against dosage induced by MDMA in isolated rat liver perfusion system Print

Corn silk is obtained from the plant Zea mays L. A traditional herbal medicine is in China. This has been used in many parts of the world to treat edema، kidney infections، gout، kidney stones، kidney diseases and prostate. Reports of the antioxidant effects of this material are available. Although little scientific resources are available to confirm its efficacy. In this study we tried to find out the antioxidant effect and preventing of hepatotoxicity effect of Corn silk with IRLP Isolated Rat Liver Perfusion) system. The aqueous and methanol extracts of dried Corn silk doses (10، 20، 40، 50 and 100 mg/kg) was used. Albino Rats weighing 220-180 g were examined after anesthesia by diethyl ether، the abdominal cavity of the animal T-shaped pattern excision in the abdomen and around) is opened. Then portal vein connected to the perfusion flow by using small scalp Vienna (No. 23) into the portal vein. After reaching perfusion flow rate to 20 ml per minute، extracts and fraction with above doses were added to perfusion buffer. Fluid outflows from the inferior vena cava، were collected for measurement of glutathione. One sample of the liver was removed for glutathione measurement and one sample was maintained in 10% formalin for histopathological examination. Differences between group means were estimated using oneway ANOVA followed by Duncan’s multiple range test. The results showed that reduced glutathione level increased significantly by aqueous and methanol extract in comparison with controls. Pathology results confirmed that by increasing dose of extracts، severity of tissue damage (hemorrhage، fibrosis، and necrosis) is reduced. In samples taken at intervals of 120 minutes، changes in the glutathione of case groups showed significant difference in comparison with the control group (p<0. 01). Findings indicated that aqueous and methanolic extracts of corn fiber، reduced hepatic damages of MDMA significantly. This effect was dose dependent and because of flavonoid and phenol compounds.