Growth Factor and Laminin Effect with Muscular Fiber Sheath on Repairing of the Sciatica Nerve

Peripheral nerve injuries which can lead to a physical disability. If the defect is very low، direct suture without tension on both ends of the cut nerve regeneration is considered as a standard procedure. Otherwise، to reconstruct the axons، the gap must be filled by graft material in order to the guidance. Due to the similarity of the matrix tubular skeletal muscle and nerve muscles graft was used to repair in this study. In the present experimental study، 42 female Wistar rats were divided into three groups and underwent surgery. In the first group a narrow strip of muscle was prepared by freezing – thawing، and later sutured between the distal and proximal sciatic nerve. In the second group، the gap caused by muscle graft was regenerated and the nerve growth factor and laminin was injected into the graft. In the control group، the two ends of the cut nerve were hidden beneath the adjacent muscles. Next، a group of rats with sciatic functional index was investigated for the behavioral. On the other group were examined for histological studies after two months. Sciatic functional index and Mean counts of myelinated fibers in two graft groups compared with the control group was significant) p<0. 05). Statistical analysis was performed using ANOVA test. co-axially aligned muscle grafts were an appropriate alternative substitute for repairing. It seems that the nerve growth factor and laminin have a positive role in axonal regeneration and functional recovery acceleration.