Bio-detoxification of AFB1in animal feeds using Saccharomyces cerevisiae

Mycotoxin contamination in human food and animal feed is a serious problem. In this research, Aflatoxin binding ability to cell wall of Saccharomyces cervisiaie was investigated in order to reduce toxicity in animal feeding. In this study, cottonseed as animal feed was contaminated with Aflatoxin B1at concentrations: 5, 10, 20 μg/lit. The contaminated feed was inoculated with yeast cell culture (heat treated, acid treated and without treatment) in an exponential phase 109 cells/ml) and incubated for 0, 4, 12, 24 hours in order to study AFB1 reduction. Result showed the average reduction of AFB1 for different treatments as follows: acid treatment (3.417 ppb), heat treatment (3.607 ppb) and viable cells (3.96 ppb).Also, the most reduction of toxin was observed within the first four hours of incubation which means that Aflatoxin binding reaches to saturation rapidly. The results indicated that yeast cells, whether viable or non viable are appropriate for detoxification of contaminated animal feeds. Moreover, binding has a physical nature. This property, especially for animal feeding and foods which have high risks of Aflatoxin contamination is considered a pertinent asset.