The Protective Effect Of N-Acetyl cysteine On Glutathione Levels And Serum Cholinesterase In Acute Poisoning Of Diazinon, in mice

Background and The wide spread usage of pesticides for improving agricultural programs is a risk factor of acute and chronic human poisoning، especially farmers. Diazinon is one of the most common organophosphates insecticides that cause oxidative stress and lipid peroxidation. The mechanism of organophosphates is postulated to be non-irreversible anticholinesterase action. This study is about the protective effect of N-Acetyl cysteine (NAC) on glutathione levels and serum cholinesterase in acute poisoning of diazinon in mice. This study was performed on 30-40gr weight male mices. The animals were randomly divided into 7 groups (n=5). The following chemicals were injected intraperitoneally: group 1 (Normal salin)، 2 (Soya oil)، 3 (Diazinon 20 mg/kg)، 4 (NAC 100mg/kg)، 5 (NAC 100mg/kg + Diazinon 20mg/kg)، 6 (Pralidoxim 20mg/kg + Diazinon 20mg/kg)، 7 (Pralidoxim 20 mg/kg + Diazinon 20 mg/kg +NAC 100mg/kg). 24 hours after last injection، the animals were sacrificed after receiving ketamine anesthesia then their liver tissues were removed. Furthurmore، Glutathione (GSH) level were determined by Elman method. After injury، 3ml blood from the heart tissue was taken with heparin syringe and cholinesterase inhibition activity was determined by Elman method. in comparison with the control group، an increased level of glutathione and blood cholinesterase was observed in the cases; although this increasing was not significant (p>0. 05). It seems that NAC has a protective effect on anticholinesterase activity and body’s antioxidant status against organophosphate pesticide، such as diazinon. The protective effect was not significant in acute poisoning. The confined opportunity of the cell to utilize cysteine as the substrate in producing glutathione may be considered as the main reason of this process.