Expression levels of mRNA cytokines of IL-17 and IL-23 in epithelialfiber of stomach inpatients with Helicobacter pylori using Real-Time PCR in Chahar Mahal and Bakhtiari province

IL-17 and IL-23 play an important role in intestinal mucosal defense against certain infections IL-17 causes neutrophils absorption to the site of infection and it results in inflammation. In this study، IL-23 and IL-17 mRNA expression levels will be checked in gastritis patients with Helicobacter pylori infection and patients without infection by quantitative Real-Time PCR Method. In this a case – control study، 58 patients with gastritis with H. pylori infection and 50 patients who were free of infection، were biopsy through endoscopy. After extracting mRNA into cDNA، mRNA IL-17 and IL-23 expression levels in samples were measured by Real-Time PCR and cytokines expression were analyzed between infected and non infected groups using the Mann–Whitney test. In this study، no significant relation was observed between IL-17 mRNA expression in patients with H. pylori gastritis and gastritis patients without infection (P=0. 941). And also no significant correlation was observed between the expression levels of mRNA IL23 in patients with Helicobacter pylori gastritis and patients without infection (P=0. 76) The results showed that expression level in IL-17 mRNA and IL-23 in patients with Helicobacter pylori gastritis compare with the patients without infection is not higher، so there is not a significant relationship between the two groups. Therefore it’s requiring to seek the role of other involved cytokines in appearing gastritis for evaluation the treatment program.