Survey of protection role of selenium in reduction of T-2 toxin effect on phagocytosis function of human neutrophil

T-2 mycotoxin belongs to Trichothecene family. This toxin has some severe effects on immune system cells. This study attempted to invesigate the T-2 toxin influence on phagocytosis function of human peripheral blood neutrophil and the protection role of selenium on this process. In this experimental study، human peripheral blood neutrophils purified using dextran-phicol method. For determining phagocytosis function of neutrophils، opsonized Candida albicans yeasts count was used. Then neutrophil phagocytosis function in presence of several concentration of T-2 toxin was investigated and numerous selenium concentrations on reduction of T-2 toxin effects were studied. Data were analized using ANOVA in Stata. T-2 toxin in 100 µg/ml concentration affected on Candida killing function of neutrophil and reduced this function to half. Selenium in 100 ng/ml had the best effect and approved Candida killing function to 90%. This study shows that T-2 toxin causes reduction of phagocytosis function of neutrophil and selenium has protection role and can return phagocytosis activity to near normal state.