Simultaneous Spectrophotometric Determination of Amlodipine and Metoprolol by Principal Componentregression Multivariate Calibration and Comparison With HPLC

Multi-drug therapy in high blood pressure is appreciated to achieve a normalblood pressure.A simple spectrophotometric method based on principal component analysis has been proposed for simultaneous determination of amlodipine (Amd) and metoprolol(Met) in pharmacutical preparations. Calibration matrix contains 3.402 µgml-1to 170.130 µgml-1and from 13.060 µgml-1to 65.3 µgml-1amlodipine and metoprolol, respectively. The proposedmethod was validated by using a set of synthetic sample mixtures and subsequently applied to simultaneousdetermination of amlodipine (Amd) and metoprololin different pharmaceutical preparations.