Comparison of Capoeta capoeta gracilis genetic populations in Madarsu and Gorganrud rivers by using of microsattelite marker

To investigate the genetic variation and population structure of Siahmahi (Capoeta capoeta gracilis), one of the native species of Iran which molecular information about it is scare, 30 samples were collected from each Madarsu river in Golestan National Park, and Gorganrud river. Genomic DNA was extracted from fin tissue by phenol-chlorophorm method. PCR was performed using microsatellite primers. Results showed that all of the 9 microsatellite primers that used in this study were polymorphic. The average number of observed and effective allele were 10.2 and 7.45 respectively, and the average of observed heterozygosity was 0.98. Both the studied populations deviated from Hardy-Weinberg equilibrium proportions at a number of loci, mostly due to the exsses of heterozygosities. The Nei genetic distance between populations was 0.139 which indicated that genetic differance among the studied population was pronounced. The data generated in this study provide useful information on the genetic variation in populations of Siahmahi for management and conservation programs of this valuable native species.