Phenotype characterizations and FRY1 and GST1 gene expression levels in response to cold stress in green mutant plant of Arabidopsis

Cold temperature is one of the important abiotic stresses limiting the growth and development in plants. Usually, plants use a complex system to cope with such stress situation. Identification of acclimation mechanisms could be an important aspect in production of cold resistant plants. In this research, the phenotype and physiological characteristics as well as gene expression levels of two key stress responsive genes FRY1 and GST1 has been evaluated in response to cold stress-induced condition in the green mutant plants following comparison with the wild type plants Ler. Consequently, 17-day-old plants have been placed in 4 ºC for a long time following assessment of various phonotypical and phonotypical characterizations including total fresh weight, root fresh weight, duration of growth and development in diverse time points. The proper statistical approaches have been employed to analyze the obtained related data by using the SAS9 software. On the basis of the obtained results, the mutant plants showed more sensitivity and a prolonged growth and development in response to cold stress conditions when compared with the wild type plants. The expression levels both FIERY1, as one of the responsive gene to abiotic stresses in Arabidopsis, and GST1, as gene marker in oxidative stress, have been measured by Q Real Time PCR. Accordingly, 17-day-old plants have been placed in 4 ºC following leave sample collection in diverse time points, RNA extraction and cDNA construction. Gene expression levels of FRY1 and GST1 considerably decreased in mutant plants. As a conclusion, more sensitivity to cold stress condition might be a result of low activity of antioxidant systems in mutant plants.