Identification and isolation of VvDREB and orthologous SbDREB2A genes from grapevine (Vitis vinifera cv. Askari) under salt stress

DREB transcription factors are one of the four independent regulation systems in plants which involves in abiotic stress signaling pathway and activation of stress responsive genes. For identification and isolation of DREB genes, one-year-old own-rooted grapevine’ were used. Potted plants were subjected to150mM NaCl during the experiment and total RNA was extracted. Specific primers were designed based on predicted DREB and SbDREB2A gene homologue in halophilic plant Salicornia brachiata. VvDREB and VvsDREB (DREB like 2C) genes were isolated from grapevine (Vitis vinifera L.’ Askari’). Bioinformatics results indicated that VvDREB gene has 85% similarity with DREB2 genes from soybean family and VvsDREB gene has 80% similarity with SbDRE 2A gene. SbDREB2A and VvsDREB proteins motif studies in ELM database showed that there are no five motifs (BRCT, MAPK, PKB, NES, NLS) in VvsDREB protein.