Triclabendazole Effect on Protease Enzyme Activity in the
Message:
Abstract:
Background
Fasciola hepatica is one of the most important helminthes parasites and triclabendazole (TCBZ) is routinely used for treatment of infected people and animals. Secreted protease enzymes by the F. hepatica plays a critical role in the inva­sion, migration, nutrition and the survival of parasite and are key targets for novel drugs and vaccines. The aim of study was to determine the protease activity of excre­tory- secretory products (ESP) of F. hepatica in the presence of TCBZ anthelmin­tic.
Methods
F. hepatica helminthes were collected and cultured within RPMI 1640 [TCBZ treated (test) and untreated (control)] for 6 h at 37 °C. ESP of treated and control were collected, centrifuged and supernatants were stored at -20°C. Protein concentrations were measured according to Bradford method. Protease enzymes activities of ESP samples were estimated by using sigma''s non-specific protease activity assay. ESP protein bands were detected by sodium dodecyl sulfate polyacryla­mide gel electrophoresis (SDS-PAGE).
Results
Mean protein concentrations in control and treated of ESP samples were determined 196.1 ±14.52 and 376.4 ±28.20 μg/ml, respectively. Mean protease enzymes activities in control and treated were 0.37 ±0.1 and 0.089 ±0.03 U/ml, respectively. Significant difference between proteins concentrations and protease enzymes activities of two groups was observed (P<0.05). SDS-PAGE showed differ­ent patterns of protein bands between treated and control samples.
Conclusion
The TCBZ reduced secreted protease enzymes activities and possibly effects on invasion, migration, nutrition and particularly survival of the parasite in the host tissues.
Language:
English
Published:
Iranian Journal of Parasitology, Volume:9 Issue: 1, Jan-Mar 2014
Pages:
107 to 113
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