Charactrization and isolation of Nocardia from clinical sample Patient suspected to active tuberculosis

Nocardia as one of the most important genera of aerobic actinomycetes, soil dwellers are that in some cases, causing dangerous and lethal infections in susceptible patients, especially patients with various immune deficiencies. A number of species belonging to Nocardia can be cause chronic pulmonary infections. In this study Nocardiosis in patients with suspected pulmonary tuberculosis in patients who were negative for culture and microscopic slides, were examined. 90 patients suspected of Mycobacterium tuberculosis with negative sputum re-provision and Nocardia isolated as pathogens were studied. Paraffin baiting technique and cultured on Blood agar medium was used for isolation of Nocardia isolates. Clinical Isolates were identified by microbiological phenotypic methods identification were determined. Six clinical isolates of Nocardia from Tuberculosis patients with negative cultures and smears were isolated. In patients with suspected tuberculosis, which are detected by culture and negative microscopic, Nocardia as a cause of chronic lung disease can be important and should be considered.