Could calf fetal spinal cord extract induce nervous system regeneration between peritoneal and abdominal muscles in rat model?

In the present study we investigate whether calf foetal spinal cord pure extract could promote regeneration of CNS neuronal cells between the abdominal wall muscles and peritoneum of rat model or no. Ten adult rats were used in this study. Anaesthesia was induced and maintained with ketamin 40 mg/kg and acepromazine 5 mg/kg with intramuscular injection. 1 ml of prepared spinal cord pure extract was injected between peritoneum and transverse muscle layer. Linea alba and skin incision were sutured routinely. Every week for 5 weeks 2 rats were euthanized and samples including injection sites were harvested and stored in 10% formalin. H&E staining techniques were used for evaluation. Two rats out of ten rats showed pyogranulomatous reactions with giant cells and inflammatory responses in the injection site, however the other eight rats did not show any inflammatory reactions in histopathological evaluation. No evidence of neuronal cells regeneration was found in our study nevertheless our homemade biomaterial from foetal spinal cord did not show any immunogenic responses after 5 weeks.