Evaluating the aluminum induced oxidative stress in erythrocyte and plasma of operating room personnel

Our Previous studics hane been shown that iron ions produce oxygen free radical reaction products in the erythrocytes. The present study is evaluating the oxidative effects of aluminum in erythrocytes and plasma proteins and decline of antioxidant power of plasma in operating room personnel. Blood obtained from operating room personnel. Erythrocytes and plasma were incubated aerobically in aluminum containing metal catalyzed oxidation (MCO) system. Total antioxidant capacity of plasma was estimated by FRAP assay (ferric reducing ability of plasma), plasma protein oxidation by carbonyl assay and structural changes in Hb were followed by spectrophotometric analysis and assessment of oxy-Hb, met-Hb and hemichrome. There was a significant relationship between optical density of Hb in 340 and 420 nm and between oxy-Hb and optical density of Hb in 420 nm(p<0.05) On the other hand, 68 to 56% decrease in total antioxidant power of plasma in control group and operating room personnel, results oxidation in plasma protein and carbonyl concentration was increased three fold. These findings may be helpful in evaluating the antioxidant status and structural changes in Hb and plasma proteins in people who are exposed to aluminum especially operating room personnel.