Resistance pattern and uptake heavy metals nickel and vanadium on bacterizes that isolated from Khark Island for metal's biological cleaning

Environmental contamination of biorecomedatoin, based using microorganisms have found spread usage today. According to diversity’s metabolic microorganism and ability of their utilize from environmental contaminant as food. Microorganism also can contamination as food and convert them to material with high permanent and low toxin. If we have appropriate selection about them, we can analysis, absorption and delete various contamination by the microorganisms. We take sample from water and sediment from 7 stations on intertidal zone in Coast of Khark Island for obtains bacteria that live in sea and decrease heavy metal such as nickel and vanadium. Collected specimen cultured in environment culture of Nutrient Agar salted and environment culture of rich sea water. We evaluate by two quantity and quality with inoculated with the liquid environment BHI Broth for measurement resistance and uptake of heavy metals nickel and vanadium. We evaluate growth rate bacteria in 300 ppm of nickel and 250 ppm vanadium by Spectrophotometer UV device and absorption atomic device evaluate measurement rate of uptake vanadium and nickel by bacteria. Then, using biochemical tests both Bacillus sp. PGS4 and Bacillus sp. PGS10 were identified that were resistant. Bacillus sp. PGS4 and Bacillus sp. PGS10 had % 80.61 and %77.99 absorption of nickel 300 ppm respectively. These organisms had%70.80 and %67.58 absorption of vanadium 250 ppm.