Study of Relationship between Genetic Pattern and Susceptibility to Terbinafine in Clinical Isolated of Trichophyton rubrum

Trichophyton rubrum is one of the most common pathogeniccause of dermatophytosis. One of the medicines which has been prescribed widely for fungal infections is terbinafine which belongs to allylamines group of antifungal agents. Recently molecular typing methods have been developed for answering the epidemiological questions and disease recurrence problems. Current study has been conducted on 22 isolates of Trichophyton rubrum obtained from patients randomly. Our aim was the investigation of correlation between genetic pattern and sensitivity to Terbinafine in clinical isolates of Trichophyton rubrum. Firstly the genus and species of isolated fungi from patients have been confirmed by macroscopic and microscopic methods, then, the resistance and sensitivity of isolates against medicine have been determined using culture medium containing defined amount of medicine. In next step fungal DNA has been extracted by RAPD-PCR (random amplified polymorphic DNA) with random sequences of 3 primers. Each primer produced different amplified pattern, and using each 3 primers differences have been observed in genetic pattern of resistant and sensitive samples using each 3 primers, but there was no bond with 100% specificity. The 12 sensitive isolates which didn’t grow in 0.1 mg concentration of medicine, also had limited growth at the low concentration of medicine..Ten resistant isolates which grew in 0.1mg/ml of medicine, in lower concentration of medicine were resisted. RAPD analysis for molecular typing of Trichophyton rubrum seems to be completely suitable.