Isolation and Biochemical Identification of Potentially Probiotic Bacteria from Barbus grypus intestine

Lactic acid bacteria (LAB) are the most common type of microbes used as probiotics. Isolation and identification of lactic acid bacteria (LAB) from Barbus grypus intestine، based on their probiotic effects، were aimed in this study. Fifty B. grypus (800. 82±265. 97) were captured from natural water resources of Khuzestan province and transferred to laboratory. The bacterial flora of fish intestine were assessed. The intestinal LABs isolated and identified according to biochemical and morphometrical tests. Isolated LABs were evaluated and ranked based on probiotical indication tests (acid resistance، bile salt resistance، bacterial antagonistic effects and lack of pathogencity in fish). A total of thirty bacillus or cocci shape bacteria، suspected LABs، were isolated. Ten isolates identified as Lactobacillus and more identified to species based on biochemical tests. The probiotical identities of these ten isolates compared and finally four respectively isolates including L. acidophilus، L. delbruecki L. brevis and L. fermentium، were selected as potentially probiotic LABs of B. grypus intestine.