Study on Genetic Diversity of Cultured Cyprinuscarpio in Khuzestan by Using Microsatellite

Aim and Common carp is one of the most important and economical species.Using artificial reproduction method, common carp is reproduced, this method can reduce genetic diversity. In the present study the genetic structure of cultured carp in Khuzestanwere studiedusing microsatellite markers. 30 specimens of common carp were collected from a farm and Shahid Maleki Reproduction Center in May2011. DNA was extracted from caudal fin tissue by phenol-choloroform-amylalcohol. PCR was performed using 4 microsatellite markers, after survey the quality and quantity of DNA by using spectrophotometer and agarose gel electrophoresis. Ho& He ranged from 0 to 1 & 0 to 0.825 respectively. Maximum number of actual allele belonged to Shahid Maleki Reproduction Center. Half of loci showed significant deviation from Hardy-Weinberg equilibrium, mostly due to the increase in heterozygosity. UPGMA cluster analysis showed there are two different populations. The results showed maximum amount of genetic diversity belonged to ShahidMaleki Reproduction Center.