Preparation of Aminophylline Anticellulite Gel and Evaluation the Effect of D-limonen and Ethanol on the Permeation Enhancement of Drug through Shed Snake Skin

This investigation aimed to prepare aminophylline gel and study the effect of ethanol and di-limonen on the permeation enhancement of drug through shed snake skin. Subjects and Aminophylline gels were prepared using theophylline and ethylene diamine as raw materials of aminophylline and hydroxypropylmethyl cellulose (HPMC) F4M and propylene glycol as gelling agent and cosolvent, respectively. Ethanol or di-limonen 5% was added as an enhancer. Drug permeability across polymeric membrane and shed snake skin was evaluated using Franz diffusion cells. Drug permeation parameters including flux (J), permeability coefficient (Kp) and enhancement factors (EFs) of each enhancer were calculated. Both accelerants significantly enhanced the absorption of aminophylline through skin model in comparison with control group (P <0.05). But they significantly reduced the drug permeability across polymeric membrane. Ethanol 60% showed a significant higher permeation effect (EF=6.61±1.05) in comparison with d-limonen 5% (EF=2.91±0.02). Both increased aminophyllin skin permeability after a lag time. D-limonen and ethanol are able to increase absorption of aminophylline through shed snake skin. Reduction in drug permeability across non-biological membrane suggests that enhancement effect on the transdermal permeation of the drug can be attributed to time-dependent reconstruction of stratum corneum.