Gene Transfer Technique Using Sperm

Nowadays، recombinant products have been produced using transgenic animal technology. So far، several techniques have been used to produce transgenic organisms. The most recent of these techniques is SMGT that has been established based on the ability of the sperm to uptake exogenous DNA and transfer it to the oocyte during fertilization. SMGT can be an efficient method when coupled with complementary methods such as electroporation، lipofection، ICSI، REMI and other techniques. The mechanism of the action of SMGT is not well defined. However، it seems that entering exogenous DNA into the sperm genome is a non-random event. The sperm chromatin condenses defines the limited sites for DNA integration. SMGT accompanied by other methods such as electroporation، lipofection، ICSI، REMI and other techniques can be an efficient method، but each of these methods has different efficiency and effects in different species. It is now clear that the sperm has the ability to transfer DNA molecules to egg during fertilization. Recent advances have reported that the efficiency of SMGT is growing and it is a cheap، fast and efficient technique for producing transgenic animals.