Tissue Cultur Studty in Foxglove Plant (Digitalis Nervosa Staud & Hochst)

Different tissue culture techniques of Digitalis nervosa staud & Hochst were studied.In this study, different kinds of plant growth regulators and sources of carbon (sucrose,galactose, fructose and maltose) were used to investigate the callus induction by leafexplants. Growth regulators 2,4-D and NAA at three levels and BA at two levels wereapplied. Murashige and Skoog culture medium supplemented with sucrose, 0.5 mgl-1BAand 1 mgl-1 NAA was found to be the best treatment for callus induction from leafexplants. In comparison with other culture media callus was appeared sooner on MSmedium containing 1 mgl-1 BA, 1 mgl-1 NAA and 2 mgl-1 2,4-D. The cotyledon explantswere the best samples for callus induction compared with the other explants (leaf,hypocotil and root). Hormones BA and NAA were used to investigate plantletregeneration from leaf based callus. Treatment containing 2 mgl-1 BA was the bestchoice to shoot regeneration from leaf explant. Murashige and Skoog culture mediumacted better than B5 for regeneration. Hormone IBA was used at two levels (0.5 and 1mgl-1) for root induction. It was determined that there is no significant differencebetween these two treatments.