Rapid Diagnosis of Pulmonary Tuberculosis From Sputum by Polymerase Chain Reaction
Message:
Abstract:
Background
Tuberculosis (TB) is a curable infectious disease yet the leading cause of death worldwide and in Bangladesh it is responsible for 7% of total deaths every year..
Objectives
This cross sectional study was conducted to isolate and identify Mycobacterium tuberculosis from sputum and to evaluate the efficacy of polymerase chain reaction (PCR) for the early diagnosis of pulmonary tuberculosis..Patients and
Methods
A total of 150 clinically suspected pulmonary TB patients (male/female: 97/53; age: 31.9 ±9.8 years) were enrolled in this study. Freshly passed single morning sputum was collected from each patient and diagnostic efficacy of PCR was compared with staining and culture methods..
Results
Among 150 sputum samples, 25 (16.7%) were positive by Ziehl-Neelsen (ZN) stain, 37 (24.7%) yielded growth in Lowenstein-Jensen (LJ) media and 45 (30%) were positive by PCR. Of the 37 isolated Mycobacteria in culture, 36 were M. tuberculosis and one was identified as non-tuberculous Mycobacterium by PCR and biochemical tests. The mean detection time was 26.9 days for the culture and 12 hours for the PCR method. Considering the culture method as the gold standard, the sensitivity of PCR was 97.3% and specificity was 92% with accuracy of 93.3%. Twenty-one (16.8%) smear negative cases and nine (7.9%) culture negative samples were positive by the PCR method..
Conclusions
PCR was the most sensitive, accurate and rapid method for diagnosis of TB. It was concluded that, for the diagnosis of pulmonary tuberculosis, PCR directly from sputum may be recommended in Bangladesh, especially in suspected tuberculosis patients who remain negative by conventional methods..
Language:
English
Published:
Archives of Clinical Infectious Diseases, Volume:9 Issue: 2, Apr 2014
Page:
7
magiran.com/p1305110  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 990,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
دسترسی سراسری کاربران دانشگاه پیام نور!
اعضای هیئت علمی و دانشجویان دانشگاه پیام نور در سراسر کشور، در صورت ثبت نام با ایمیل دانشگاهی، تا پایان فروردین ماه 1403 به مقالات سایت دسترسی خواهند داشت!
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 50 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!