Immunological Analysis of Aerobic Bioreactor Bovine Theilerio¬sis Vaccine

In this study, the pilot production of aerobic bioreactor tropical theilerio­sis vaccine was optimized with the aim of immunological assays for further mass production. We have shown earlier the delayed type hypersensitivity (DTH) assay could be used for evaluating the immunity and memory cells against specific Theil­eria antigen in vaccinated animals. In addition, TNF-α is the principle cytokine in modulating the cytotoxic activity of cytotoxic T-lymphocytes (CTL). Immunologi­cal analysis of the vaccine was performed by using two cell mediated immunity (CMI) in vitro and in vivo DTH test (Theilerin) and TNF-α assay. The results of immune responses of susceptible immunized cattle by bioreac­tor vaccine in comparison with conventional flask vaccine revealed a signifi­cant stimulation of immune cells by transcription of high level of TNF-α and posi­tive reaction against Theileria antigen in Theilerin skin test (DTH). The equal immunological results achieved in both above mentioned vaccines verified the satisfactory immunity for aerobic bioreactor theileriosis vac­cine for advance mass vaccination in the field on a large scale.