A Review on Incidence of Alloimmunization with Incompatible Rh Blood Group Transfusion and Role of Molecular Methods in Finding Compatible Blood Units in Multi Transfused Patients

Alloimunization reduces RBCs lifespan in circulation. Identification of phenotypes of RBCs could be helpful in reducing the rate of alloimunization. But often, due to persistence of donor RBC`s in patients circulation, precise identification of blood group antigens in multi transfusion patient would be difficult. Today, DNA technology increased our knowledge in recognizing the molecular basis of blood group antigens. This knowledge helps in predicting profiles of blood groups in patients and overcoming problems of agglutination method. But theoretically, it seems, because of contamination of donor`s WBCs in patients circulation and possibility of their contamination with patient`s WBCs, application of molecular method by WBCs may not be reliable. However, some studies showed that using patient's WBCs can infinitively be applicable in identification of polymorphism of blood group antigens by molecular methods, even in newly transfused patients.