Phylogenetic study of Peronia peronii species (Mollusca; Gastropod: sea slug) in inter-tidal Chabahar coast, based on 18s-rDNA sequence
Message:
Abstract:
Aim and
Background
Phylogenetic analysis and morphological identification on species of each region can be used as the basis for establishing subsequent studies. Many phylogenetic studies have been carried out according to mitochondrial genome on gastropods group in other coastal areas of the world but there are not much data on nuclear genome. Most Gastropod study in Iran is founded on morphology of the species, and there are no records of molecular study from south coast of Iran. In this study phylogenetic analysis of a sea slug species from Chabahar coast was examined for the first time.
Materials And Methods
Samples were collected from rocky shores of Tis in Chabahar and transferred to the laboratory. The samples were analyzed morphologicaly prior to being frozen in freezer -80 degrees. DNA was extracted by using CTAB method and then Polymerase Chain Reaction, sequencing alignment were conducted and phylogenic tree was obtained.
Results
Nucleotides sequence of the gene in 18s rDNA region was analyzed. The phylogenetic analysis was conducted and found that the Iranian species and other species in Panpulmonata clad have more divergence than species of the other two clads.
Conclusion
Maximum likelihood phylogenetic analysis showed that the Iranian species from Chabahar coast is in the Panpulmonata clad and with 100% bootstrap support is a sister group Peronia Cf.peroni. The molecular results are confirmed by morphological features.
Language:
Persian
Published:
New Cellular & Molecular Biotechnology Journal, Volume:4 Issue: 15, 2014
Pages:
29 to 34
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