Genetic comparison of Oxynoemacheilus Kiabii (Golzarianpour, Abdoli and Frehof 2011) from Gamasiab River in Kermanshah and Hamadan Province, using microsatellite markers

Six highly variable microsatellite loci were used to investigate the genetic diversity and population structure of the Oxynoemacheilus kiabii in the Gamasiab River in between the Hamedan and Kermanshah provinces. A total of 60 samples were collected from these areas. All of the 6 microsatellite loci screened in this study showed polymorphism. A total of 70 alleles were observed for six loci. The number of alleles per locus ranged from 6 to 16. The averages of observed (Ho) and expected heterozygosity (He) recorded as much as 0.515 and 0.849, respectively. Most cases deviated significantly from Hardy-Weinberg equilibrium (p≤0.01). It seems that there is a low differentiation between two populations of this species through the studied areas due to high level of gene flow (Nm=14.554) between two regions and low Fst (0.021).