Culturable prokaryotic diversity of Urmia salt lake
Message:
Abstract:
Microbial life is present not only in our familiar world but also in extreme environments. Salt lakes with near or at saturating salinity are extreme environments that common all over the world. The study in detail of such environments would permit to determine not only the microbial diversity but also the gene pools and potential use of this information for biotechnological applications. Urmia Salt Lake in the northwestern of Iran is the second saltiest lake in the world and resembles the Great Salt Lake in the western USA. Water, soil, sediment and salt samples were taken from east and western sites in Urmia Salt Lake in July 2012. Direct plating, dilution plating and long incubation period were used to isolate organisms on MGM, MH, SWN medium. Isolates were taken from the samples by using the conventional culture-dependent methods. Of these, 36 isolates were selected for sequencing and phylogenetic analysis, based on their growth characteristics and colony morphology. Two hundred and twenty-eight of microorganisms were obtained from soil, salt, water and sediment samples collected from the east and western of the lake. Of these, 36 isolates were selected for sequencing and phylogenetic analysis. Results showed that 36 strains represented 8 species, belonging to 3 genera Halorubrum, Haloarcula, Haloterrigena. As total, bacterial isolates were belonged to Salicola, Pseudomonas. All strains showed 96.5 to 100 % similarity in 16S rDNA sequencing. Of these, 5 strains showed less than 98.7% sequence similarity to the closest known strains and were representatives as new taxa of Urmia Lake.The phylogenetic analysis of sequences of Urmia Lake indicated in overlaps with 16S rDNA sequences from other lakes with similar habitats.
Language:
Persian
Published:
Journal of Genetics, Volume:9 Issue: 3, 2014
Page:
313
magiran.com/p1313842  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 990,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
دسترسی سراسری کاربران دانشگاه پیام نور!
اعضای هیئت علمی و دانشجویان دانشگاه پیام نور در سراسر کشور، در صورت ثبت نام با ایمیل دانشگاهی، تا پایان فروردین ماه 1403 به مقالات سایت دسترسی خواهند داشت!
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 50 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!