Genetic diversity in bread wheat (Triticum aestivum L.) as revealed by microsatellite markers and association analysis of physiological traits related to spring cold stress
Author(s):
Abstract:
Genetic diversity is a necessary factor for breeding programs and increasing genetic gain of selection in crop plants. Molecular markers have been successfully used for analysis of genetic diversity in various crops. For this purpose the 20 bread wheat cultivars were in a completely randomized design and five physiological traits, total chlorophyll, chlorophyll a, chlorophyll b, carotenoids and their stability was assessed in the cytoplasm membrane. Study of genetic diversity accomplished by 12 SSR marker primers. In order to determine genetic relation between cultivars, cluster analysis in Ward method was performed and cultivars were into four groups. The association between 40 microsatellite bands of 12 primer pairs and 5 physiological traits recorded on 20 wheat bread cultivars. Polymorphic information contents (PIC) of loci were in the range of 0.32 (Xgwm10) to 0.725 (Xgwm33). By multiple regression stepwise relationship of each 5 physiological traits and the 40 polymorphic markers were studied. The result of stepwise regression analysis showed the significant relationship of 2 markers with 5 physiological trait at the contort temperature and also 6 marker with those physiological marker at the severe level of spring cold stress, may facilitate preliminary selection of these traits for breeding purposes. Moreover, our results showed that some of the markers were related to more than one trait, implying close linkage and/or peliotropy of these genes on chromosomes.
Keywords:
Language:
Persian
Published:
Journal of Genetics, Volume:9 Issue: 3, 2014
Page:
279
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