Surveying of antibiotic resistance pattern and frequency rate of blaTEM in the ESBLs producing E. coli isolated in Rasht

E. coli is one of the most frequent causes of nosocomial infection. Antimicrobial resistance leads to failure in treatment of hospital infections caused by E. coli. Extended-Spectrum Beta-lactamases (ESBLs) is one of the causes antibiotic resistance in this bacteria. The enzymes are predominantly plasmid mediated and are derived from TEM and SHV type enzymes. The aim of this study was to investigate the frequency of blaTEM genes in ESBL-producing E. coli strains isolated from admitted patients in six hospitals in Rasht, Iran. All 160 clinical samples have been taken from various samples of hospitalized patients after diagnosis of E. coli, antibiotic resistance was surveyed by Kirby-Bauer method. For resistant isolates, double disk phenotypic confirmatory test was carried out in order to diagnosis ESBL-producing strains. Then from ESBL-producing strains, DNA extracted And blaTEM genes were detected using PCR. Among 160 clinical isolates of E. coli which were collected, the maximum resistance to Amoxicillin has been observed in all strains, and all were susceptible to Imipenem, and 51.9% of strains were ESBL positive, that 27 strains (32.5%) of blaTEM genes were observed using PCR.Discussion & In this study, more than 50% were detected as ESBL-producing strains, which More than half of the isolates were blaTEM positive. Comparing these results with other studies in this field shows a direct relations between development of resistance to beta-lactam antibiotics and genes transfer such as bla TEM.