The effects of different concentrations of Acid ascorbic on freezability of Kordi Goat Spermatozoa

This study was designed to investigate the effects of different concentrations of ascorbic acid (0، 2. 5، 4. 5، 6. 5 and 8. 5 mg/ml) on freezing of the Kordi goat spermatozoa. Semen samples were collected by an artificial vagina، twice a week from four pubertal goat. The extender containing semen was frozen in liquid nitrogen and then was stored until using for assessment. Semen was thawed at 37◦C and then motility and progressive motility were assessed by CASA. membrane integrity، viability and morphology of sperms were assessed and malondialdehiyde (MDA) concentration was measured. The results of this experiment showed that extender containing 4. 5 and 6. 5 mg ascorbic acid significantly improved motility (P<0. 01)، progressive motility (P<0. 05) and viability compare to control treatment (P<0. 01). Membrane integrity، morphology and malondialdehiyde were not affected significantly by the different level of ascorbic acid (P>0. 05). It can be concluded that adding 4. 5 mg/ml ascorbic acid in to goat extender is suitable for preservation of sperm and its fertility.