Effects of Intracerebroventricular Administration of Salubrinal-A Protein Synthesis Inhibitor-on Epileptic Seizures Induced by Pentylenetetrazol in Male Rats

Salubrinal- a protein synthesis inhibitor- plays a crucial role in sleep and neuronal inhibition through the GABA activation. According to these evidences, the aim of the present study was to determine the effect of salubrinal on epileptic seizures induced by pentylenetetrazol (PTZ). In this expremental study under stereotaxical surgery, three monopolar electrodes were fixed on the skull bone of animals and a cannula was implanted in the right cerebral ventricle according to Paxinos coordinates. After 7 days of recovery, each animal received intracerebroventricular (ICV) vehicle, diazepam (0.2 mg/rat) or salubrinal (25 or 50 µg/rat) 10 minutes before Pentylenetetrazol injection (subcutaneous, 60mg/kg). Electrophysiological and behavioral parameters of induced seizures were recorded during 60 minutes. Salubrinal (25 and 50 µg/rat) significantly retarded the onset of generalized tonic-clonic seizures when compared to vehicle treatment (p<0.05). Epileptic discharge duration and ictal discharge duration were significantly shortened in salubrinal treated rats (p<0.05). Additionally, the rats treated with salubrinal (25 and 50 µg/rat) had lower seizure stage scores, as compared to vehicle treated rats (p<0.05). The findings of this study revealed that shortly after ICV. administration of salubrinal, epileptic seizures can be significantly decreased. Also, the findings suggest that protein synthesis is an essential process during development of epileptic seizures.