The effects of therapeutic dose of oxytetracycline on magnesium, calcium and parathormone in sheep
Message:
Abstract:
Infertility due to heat stress is one of the great economic problems in dairy industry. Heat stress at the earliest stages of follicular growth can affect the oocyte quality and developmental competence. The objective of this study was to determine factors which may have role to reduce of polyspermy in heat stress condition. For this propose, some changes in sperm concentration, maturation medium, and fertilization medium were done. In first step, three concentrations of sperm (0.5×106, 0.75×106 and 1×106) were selected. Results showed that with decrease in sperm concentration, polysermic rate did not significantly changed (p>0.05). But in 0.5×106 sperm concentration, fertilization rate significantly decreased (p<0.001). So, for next steps 0.75×106 sperm was used. In second experiment, fetal bovine serum in two concentrations (10 or 20%) was added to maturation medium. Result did not show any difference between these two groups in polyspermic rate, normal fertilization, and total zygote (p>0.05). In the next step, adding two different levels of heparin (1 or 10 μg/ml) in fertilization medium was evaluated. The fertilization parameters did not affected by both concentrations of heparin (p>0.05). In last experiment, non-essential amino acid was removed from fertilization medium. Polyspermic rate was reduced to less than 15% in non-amino acid group. The cleavage, blastocyst, hatched blastocyst rates significantly promote in group without amino acid (p<0.05).
Language:
Persian
Published:
Iranian Journal of Veterinary Clinical Sciences, Volume:7 Issue: 2, 2013
Page:
63
magiran.com/p1334102  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 990,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
دسترسی سراسری کاربران دانشگاه پیام نور!
اعضای هیئت علمی و دانشجویان دانشگاه پیام نور در سراسر کشور، در صورت ثبت نام با ایمیل دانشگاهی، تا پایان فروردین ماه 1403 به مقالات سایت دسترسی خواهند داشت!
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 50 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!