Evaluation of curcumin effect on renal ischemia- reperfusion injury in dog

Fifteen healthy adult male dogs were divided into 3 groups: vitamin C (Vit C), glycerol formal (GF), and curcumin (Cur). Fifteen days after right nephrectomy, the left kidney vessels were exposed. Vit C (100mg/kg), GF and Cur (10mg/kg) were administered intravenously Ten minutes before ischemia. Clamps were removed After 45 minutes of ischemia and the animals were euthanized 48 hours thereafter. The kidney was removed immediately and tissue malondialdehyde (MDA), catalase (CAT) and thiol (-SH) levels were determined. Histopathological examinations were also performed. Percentage of tubular necrosis in group Vit C, GF and Cur was 7.12±1.86, 8.35±2.26 and 4.62±2.28 respectively. There was no significant difference in tubular necrosis (P≤0.05). Urinary casts in GF group was significantly more than other groups (P≤0.05). Tissue CAT activity and MDA levels was significantly further in Vit C and GF group respectively (P≤0.05). Furthermore, there was no significant difference in tissue Thiol levels between three groups (P≤0.05). In conclusion, the administration of curcumin has limited protective effects on main renal ischemia- reperfusion injury.