Antimicrobial Susceptibility Patterns and Molecular Epidemiology of Pseudomonas Aeruginosa Isolated from Burn Patients
Message:
Abstract:
Background and Aim
Because of emerging multi-drug resistance (MDR) Pseudomonas aeruginosa strains, treatment of burn patients infected by this bacterium is difficult. The aim of this study was to detect antimicrobial profile and molecular epidemiology of metallo-beta-lactamase (MBL) producer strains.
Methods
In this cross-sectional investigation 270 Pseudomonas aeruginosa isolates were collected from the burn patients. Carbapenem sresistance strains were detected by phenotypic E-test method. Susceptibility profiles of metallo-β-lactamase (MβL) enzyme producing isolates of this bacterium to 11 antimicrobial drug were determined by disc diffusion method according Clinical and Laboratory Standards Institute (CLSI) guidelines. The genetic correlations between isolates were determined by Pulsed-Field Gel Electrophoresis (PFGE) method.
Results
Among 270 P. aeruginosa isolates, 60 (22.2%) strains showed resistant to meropenem (MEM) and imipenem (IMI) and were considered as metallo-β-lactamase positive. All metallo-β-lactamase positive isolates were resistant to five tested antimcrobial while their sensitivities to the three best effective antibiotics including ciprofloxacin, amikacin and ceftazidime were 1.7%, 6.7 % and 23.3%, respectively. Majority of the isolates (71.6%) showed more than 80% similarity based on the drawn dendrogram.
Conclusion
Our results showed, the tested antimicrobials are not safe to prescribe for burn patients. According PFGE pulsotypes, a limited number of P.aeruginosa types are common in the hospital burn unit which infect the patients hospitalized in this ward.
Language:
Persian
Published:
Armaghane-danesh, Volume:19 Issue: 8, 2014
Page:
694
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