Genetic diversity of fluorescent pseudomonads isolated from wheat fields with biocontrol ability against Gaeumannomyces graminis var. tritici

In this research different isolates of fluorescent pseudomonads was provided and after investigation of their biochemical characteristics، and their ability to control take-all disease of wheat، their genetic diversity and relation between DNA fingerprinting and biocontrol ability of the isolates was investigated using rep-PCR molecular marker. DNA fingerprinting results and the drown dendrogram showed that19 fluorescent pseudomonads isolates differentiated in 25-100% genetic similarity level and the isolates belonged to nine fingerprinting groups assigned by letters A-I. Based on these results، there was a relation between DNA fingerprinting and inhibition zone، production of HCN and diacetylphloroglucinol production bioassays. Therefore، we suggested that rep-PCR molecular marker is a useful tool for identification and screening of fluorescent pseudomonads with biocontrol ability.