The Prevalence of Infectious Bronchitis in Respiratory Syndrome in Broiler Chicken Flocks of Chaharmahal va Bakhtiyari Province
Avian infectious bronchitis (IB) is caused by corona viruses. Infectious bronchitis virus (IBV) is a major cause of economic losses in poultry and can be involved in respiratory disease، nephritis، and both poor egg production and quality by reproductive tract infection. IBV has many serotypes that do not confer cross protection against each other. The study in 2012 was conducted to detect IBV in broilers chicken farms suspected of respiratory syndrome with a high mortality and no history of vaccination against of the IBV in Charmahal va Bakhtiyari province. In this study، one hundred serum samples and one hundred tracheal and lung tissue samples were collected from 10 broiler chicken farms with respiratory signs. To achieve this goal، two tests have been used throughout the studies of protocol Enzyme-Linked Immunosorbent Assay (ELISA) and Reverse Transcription Polymerase Chain Reaction (RT-PCR). The presence of IBV antibodies was detected by using ELISA kit. The results indicated that 7 flocks (70%) were positive and 3 flocks (30%) were negative tested with RT- PCR method. The evaluation of serum antibody titers of flocks showed that antibodies against infectious bronchitis virus in RT-PCR positive flocks were higher than RT-PCR negative flocks. The results also evinced that the prevalence of infection bronchitis in broiler flocks was high in broilers farms of Chaharmahal va bakhtiyari province and concurrent Infection of IBV and other respiratory pathogen played a role in high mortality rate of respiratory disease.
Journal of Veterinary Microbiology, Volume:10 Issue:2, 2015
75 - 82
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