Rapid identification of Pseudomonas species in reared Hypophthalmichthys molitrix in Guilan province
Message:
Abstract:
Pseudomonas spp. are zoonotic bacteria associated with human and fish accompanied to hemorrhage in the fish. Rapid detection of this disease leads to in time treatment. Present study was performed aiming to compare two kits including API 20 NE and API 20 E with routine biochemical tests to detect pseudomonas species. With occurrence of serious losses in the reared Hypophthamichthys molitrix in Guilan province during spring and summer when environmental conditions were suitable for bacterial infection outbreak، 126 fish from 25 warm water farms locating in Rasht were transferred to the fish health and disease department of Inland water aquaculture institute. After carefully dissection، Samples of kidney، liver، spleen and ascetic fluid of each fish were aseptically streaked onto blood Agarthen incubated for 24-48 hours at 30 ºC. Simultaneously، routine biochemical techniques and API kits were used for characterization. Antibiotic resistance pattern for all pseudomonas isolates was determined using disk diffusion technique on Moller- Hinton agar according to Bauer، et al method (1966) modified based on NCCLS (2007). Based on the results of 75 isolates، 9 isolates (12%) were pseudomonas spp.. Nine isolates were placed in 2 species of P. aeruginisa and P. fluoresences using API 20NE with 98% accuracy While API 20E detected P. aeruginisa with 64% accuracy and P. fluoresences was defined as P. fluoresences/putida. In the antibiotic sensitivity test، gentamycin was the choice drug for both species and for P. fluoresences، Kanamycin and neomycin also had 100% influences. Results indicated that among 2 API systems، API 20NE strip is capable to detect pseudomonas species in fish with higher precision and dissociation ability compared to API 20E.
Language:
Persian
Published:
Journal of Veterinary Microbiology, Volume:10 Issue: 2, 2015
Pages:
93 to 102
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