A Study on the Staphylococcus pasteuri Deoxyribonuclease Activity
Message:
Abstract:
All organisms have nuclease. Deoxyribonucleases (DNase) are from enzyme group that are able to hydrolase phosphodiester bounds of DNA molecule bonds. Staphylococcus pasteuri identified by 16S rRNA marker and biochemical tests، and submitted to GenBank/EMBL with KC170006 accession number. The activity of DNase enzyme was detected through three spectrophotometric، detection of DNase activity on circular and double strand DNA molecule on agarose gel methods. The effects of pH، NaCl and cations like Mg2+، Ca2+،Mn2+andMg2+-Ca2+ treatments were considered. S. pasteuri DNase was able to cut single strand of circular DNA. S. pasteuri DNase was able to cut single strand DNA. Studies on circular DNA showed that the enzyme may nick specifically، whereas pET-21a (+) was restricted to one point. The DNase does not cut double strand DNA. This is the first report about S. pasteuri DNase activity. The most important activity of enzyme was observed in the presence of 0. 6 M NaCl، 1mM Mg2+-Ca2+ and pH 5. 5.
Language:
Persian
Published:
Journal of Veterinary Microbiology, Volume:10 Issue:2, 2015
Pages:
143 - 153
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