New method (biotechic) for artificial breeding of Barbus sharpeyi

Barbus sharpeyi in family Cyprinidae is an endemic fish of the province Khuzestan of Iran. These fish are produced by artificial breeding using carp pituitary extract (CPE). According to the problem of artificial breeding about this fish in Iran، the objective of this study was to obtain the effectiveness new method (LHRH-a2 hormone Combined with carp pituitary extract (3 injections) on reproduction index in Barbus sharpeyi. The results showed that the LHRH-a2 hormone combined with carp pituitary extract in three injection (specially treat2) lead to high spawning success (62% average) and weight of stripped egg mass/ weight of stripped egg mass (8. 49% average) and fertilization success (76. 57% average). Therefore، it can be concluded that like many other cyprinids، LHRH-a2 hormone combined with carp pituitary extract (3 injection) can be effectiveness for spawning induction in Barbus sharpeyi.