Nematicidal Activity of Essential Oils of Ecalyptus spp., Dorema ammoniacum and Ferula galbanifula on Root-knot Nematode (Meloidogyne javanica) In vitro

Root-knot nematodes (Meloidogyne spp.) are one of the most important plant parasitic nematodes which cause damage to crops. In recent years، using of medicinal plants has been considered to many scientists as effective methods for controlling nematodes. In this study، essential oils of Ecalyptus spp.، Dorema ammoniacum and Ferula galbanifula were tested against the root-knot nematode (Meloidogyne javanica) in vitro conditions. Essential oils were obtained by hydro-distillation using a Clevenger-type apparatus. Five concentrations of each oil were used with three replications. The results was indicated direct relationship the degree of hatching inhibition and juvenile mortality with concentration oils. The essential oil of Ecalyptus spp. proved to be most toxicant with the LC50 values of 839 and 2122 ppm against eggs and juveniles، respectively. At the highest concentration (4000 ppm)، degree of hatching inhibition by oil of Ecalyptus spp.، D. ammoniacum and F. galbanifula were recorded 98. 5، 93. 9 and 90. 3%، respectively. At the same concentration، juveniles mortality were obtained 98. 8، 66. 7 and 58. 7%. The results demonstrated that the essential oils of these plants particularly، oil of Ecalyptus spp. can be considered as potential protestants against M. javanica.