Effect of Mouse Embryo Tail Bud Co-culture on In vitro Maturation of Mouse Oocyte
Author(s):
Abstract:
Background and
Purpose
In vitro maturation of oocyte is a promising technology in treatment of infertility, however, its clinical application is still limited due to poor success rate. This study was devised to evaluate the effect of mouse embryo tail bud co-culture on maturation of immature mouse oocyte. Materials And Methods
Immature oocytes were recovered from NMRI female mice 48hr after injection of 7.5 IU PMSG (pregnant mare serum gonadotropin). Oocytes were divided into four groups in α-MEM medium containing 10% FBS. The maturation medium for positive control group contained hCG, while for negative control group it was without hCG. In experimental groups 1 and 2 the maturation medium contained no hCG, but 9.5-10.5 and 12-13 days mouse embryo tail bud. The oocytes in each group were cultured in CO2 incubator. The oocytes maturation was recorded under an invert microscope after 24 hr. Data analysis was performed applying ANNOVA in SPSS. Results
In vitro maturation rate in positive control group, negative control group, experimental groups 1 and 2 were 52, 48, 66 and 50%, respectively. These rates indicated a significant increase in matured MII oocytes (in the presence of 9.5-10.5 days mouse embryo tail bud) than those of the negative control group and the second experimental group (P<0.05). Conclusion
Co-culture of oocyte with 9.5-10.5 days mouse embryo tail bud, increased the maturation rate of immature oocyteKeywords:
Language:
Persian
Published:
Journal of Mazandaran University of Medical Sciences, Volume:24 Issue: 120, 2015
Pages:
88 to 99
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