Antagonistic activity of Streptomyces gresiofuscus PTCC1628 against isolated Gram negative bacteria from Urinary Tract Infections

Antibiotics are the best known products of actinomycete. Over 5,000 antibiotics have been identified from the cultures of Gram positive and Gram-negative organisms, and filamentous fungi, but only about 100 antibiotics have commercially been used to treat human, animal and plant diseases. The genus, Streptomycete, is responsible for the formation of more than 60% of known antibiotics. Bacterial urinary tract infections are frequent in the outpatient as well as in the nosocomial setting. The common bacteria from UTIs were isolated from hospital and laboratory samples. The present study was designed to isolate Gram-negative bacteria from urinary tract infections and evaluate Streptomyces gresiofuscus PTCC1628 antimicrobial activity against pathogenic bacteria. Bacterial isolates were identified as Acinetobater spp. and Pseudomonas aeruginosa. The antimicrobial activity was examined by the agar Well diffusion method. The cell free supernatants of the S.gresiofuscus were able to inhibit the growth of all human pathogens (Acinetobacter spp. and P.aeruginosa) isolated in this study in Well diffusion method. The isolates also showed very promising activities against multi drug resistant human pathogens. Concentrations of produced compounds by S.griseofuscus PTCC1628 were determined by GC-MS method.