Comparison of the Inhibitory and Antibacterial Effect of Aqueous and Ethanolic Extract of Kelussia odoratissima on Some Pathogenic Bacteria
Message:
Abstract:
Background And Objective
Nowadays, the increasing use of antibiotics, or failure to comply the recommended dose by the patients, has led to the development of bacterial resistance to antibiotics. It has resulted in increasing the interest to use more efficient new anti-microbial compounds without toxicity, such as medicinal plants. The aim of this study was to compare the antibacterial activity of aqueous and ethanolic extracts of Kelussia odoratissima on some pathogenic bacteria.
Materials And Methods
In this laboratory study, the antibacterial effect of aqueous and ethanolic extracts of Kelussia odoratissima leaves were investigated using pour plate and disk agar diffusion tests. Minimum Inhibitory Concentration (MIC(and Minimum Bactericidal Concentration (MBC) were also studied using the Dilution method. Analysis of results was performed using One-way analysis of variance (ANOVA).
Results
MIC of aqueous and ethanolic extracts for Staphylococcus epidermidis and Streptococcus pyogenes was 32 and 16 mg/ml and for Pseudomonas aeruginosa was 64 and 32 mg/ml, respectively. MBC of aqueous and ethanolic extracts for Staphylococcus epidermidis and Streptococcus pyogenes was 64 and 32 mg/ml and for Pseudomonas aeruginosa was 128 and 64 mg/ml, respectively. The results of One-way analysis of variance showed, with increasing concentrations of ethanolic and aqueous extracts, inhibition zone was significantly increased.
Conclusion
The results showed that the ethanolic extract of Kelussia odoratissima leaves had greater inhibitory effects on the strains studied in comparison with to aqueous extracts in vitro.
Language:
Persian
Published:
Journal of Rafsanjan University Of Medical Sciences, Volume:13 Issue: 9, 2014
Pages:
775 to 784
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